02 July 2004
Granulocyte-macrophage colony-stimulating factor (GM-CSF) in children with acute immune thrombocytopenic purpura
Elham A. Rizk, Wessam M El-Gendy, Hoda Hassab, Lamyaa El-MadboullyMed Sci Monit 2004; 10(7): CR330-335 :: ID: 11694
Abstract
Background:The purpose of our study was to assess the serum level of GM-CSF in children with acute ITP and its correlation with clinical parameters and with the level of platelet-associated immunoglobulins.Material/Methods:Thirty children with acute ITP were selected from the inpatient department at Alexandria University Children’s Hospital at El Shatby. Ten apparently healthy children served as controls. ITP Patients were divided according to platelet count into those with platelet count >20,000/ul and cases with platelet counts >20,000/ul and cases with platelet counts <20,000/ul. Serum GM-CSF was measured by the ELISA technique. Total IgG was measured by radial immunodiffusion plates, and PAIgG was detected directly by flow cytometry.
Results: Total IgG showed significant elevation in ITP patients as compared to controls (t=2.748, P<0.05), but no correlation was detected between platelet antibodies and total IgG (r=–0.140, P=0.460). 86% of the patients had elevated platelet-associated immunoglobulin (PAIgG). No correlation between PAIgG and platelet count was detected (r=–0.072, P=0.72). Serum GM-CSF was significantly higher in ITP patients than controls (t=3.757, P<0.05). An inverse correlation was found between serum GM-CSF and platelet count (r=–0.4643, P=0.010). There was positive correlation between serum GM-CSF and PAIgG (r=0.4224, P=0.020).
Conclusions: Our results suggest that GM-CSF may have a role in the pathogenesis of ITP through activation of the mononuclear phagocyte system, resulting in accelerated destruction and phagocytosis of antibody-coated platelets and decreased platelet count.
Keywords: Adolescent, Autoantibodies - immunology, Blood Platelets - immunology, Blood Platelets - metabolism, Case-Control Studies, Child, Child, Preschool, Contusions - epidemiology, Contusions - etiology, Granulocyte-Macrophage Colony-Stimulating Factor - blood, Immunoglobulin G - blood, Immunoglobulins - blood, Immunoglobulins - immunology, Platelet Count, Purpura, Thrombocytopenic, Idiopathic - blood, Purpura, Thrombocytopenic, Idiopathic - complications, Purpura, Thrombocytopenic, Idiopathic - immunology, Statistics, Adolescent, Autoantibodies - immunology, Blood Platelets - metabolism, Case-Control Studies, Child, Child, Preschool, Contusions - etiology, Granulocyte-Macrophage Colony-Stimulating Factor - blood, Immunoglobulin G - blood, Immunoglobulins - immunology, Platelet Count, Purpura, Thrombocytopenic, Idiopathic - immunology, Statistics as Topic
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952