02 July 2004
The en 455-3 modified lowry assay does not yield a reliable estimate of the allergenicity level of latex gloves with low total protein content
Carine Audo, José Barbara, Habib Chabane, Martine Armange, Francisque LeynadierMed Sci Monit 2004; 10(7): PI81-86 :: ID: 11701
Abstract
Background:Estimation of the allergenicity of latex gloves by measurement of total extractable protein content with the modified Lowry method is not satisfactory. Therefore, complementary methods that are accurate, sensitive, and clinically relevant are needed. The Competitive Immunoassay for Antigenic Latex Proteins (CIALP) method described in a previous study could be a reliable complementary method for estimating the allergenicity of latex gloves.Material/Methods:Extracts from 62 powdered or powder-free gloves (16 surgical and 46 examination) were tested by the EN 455-3 modified Lowry assay, IgE-inhibition, and CIALP. The results were compared with those from 36 glove extracts reported in a previous study.Results:Significant correlations were observed between CIALP, IgE-inhibition, and the Lowry assay for the 62 glove extracts (r between 0.79 and 0.87; p<0.001), mostly rich in proteins. After inclusion of results from the previous study, significant correlations between the three methods were again observed (r between 0.76 and 0.90; p<0.001). However, there was no correlation between CIALP or IgE-inhibition and results of the Lowry assay for gloves that had a total protein content <50 µg/g of glove. Furthermore, 15/16 extracts with undetectable total proteins were positive in both the CIALP and IgE-inhibition methods.
Conclusions: The modified Lowry assay alone is useful for estimating the allergenicity of latex gloves when the total protein content is ł50 µg/g of glove. For gloves with a total protein content <50 µg/g of glove, complementary methods such as CIALP are necessary.
Keywords: Allergens - analysis, Enzyme-Linked Immunosorbent Assay, Gloves, Protective, Immunoassay - methods, Immunoglobulin E - immunology, Latex - adverse effects, Latex - immunology, Latex Hypersensitivity - immunology, Latex Hypersensitivity - prevention & control, Plant Proteins - analysis, Plant Proteins - immunology, Sensitivity and Specificity, Statistics, Allergens - analysis, Enzyme-Linked Immunosorbent Assay, Gloves, Protective, Immunoassay - methods, Immunoglobulin E - immunology, Latex - immunology, Latex Hypersensitivity - prevention & control, Plant Proteins - immunology, Sensitivity and Specificity, Statistics as Topic
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952