01 August 2004
Growth hormone enhances gastric ulcer healing in rats
Stefan Beckert, Nicole Class, Farshid Farrahi, Stephan CoerperMed Sci Monit 2004; 10(8): BR255-258 :: ID: 11740
Abstract
Background: Gastric ulcer healing requires the reconstitution of epithelial structures and underlying connective tissue through cellular proliferation, migration, and differentiation. The systemic application of growth hormone (GH) has shown anabolic effects in postoperative and burn therapy by increasing protein synthesis and attenuating protein catabolism. There is also evidence that GH stimulates cell proliferation and differentiation. In this study we evaluated the impact of GH on gastric ulcer healing.
Material/Methods: Gastric ulcers were induced with a cryoprobe in male Wistar rats (285±11 g). The first group of rodents (n=10) received a daily subcutaneous dose of 2.5 mg/kg growth hormone for seven days. The second group (n=10) received only vehicle. After 7 days, ulcer size was determined photoplanimetrically. Cell proliferation and new vessel growth in the ulcer margin were evaluated by quantitative immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA), respectively.
Results: The systemic application of GH caused a significant increase in body weight (332±7 g vs. 289±13 g; p=0.007). Ulcer size was also reduced significantly compared with controls (5.6±0.8 mm2 vs. 9.9±1 mm2; p=0.005). Immunohistochemical analysis revealed a significant increase in cell proliferation (79.7±0.9% pos. cells vs. 64.7±1.9% pos. cells; p=0.0001) as shown by PCNA expression, and a significant increase in new vessel growth as demonstrated by SMA expression (1762±124 cells/mm2 vs. 1067±77 cells/mm2; p=0.0001).
Conclusions: Growth hormone accelerates gastric ulcer healing by stimulating cell proliferation and angiogenesis.
Keywords: Cell Division - drug effects, Gastric Mucosa - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects, Cell Division - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
18 Mar 2024 : Clinical Research
Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative StudyMed Sci Monit In Press; DOI: 10.12659/MSM.944136
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
Most Viewed Current Articles
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952