15 May 2002
Gastric hyperemic response induced by acid back-diffusion in rat stomachs following barrier disruption - relation to vanilloid type-1 receptors.
Masato Nakashima, Shigeru Kagawa, Kimihito Tashima, Shinichi Kato, Koji TakeuchiMed Sci Monit 2002; 8(5): BR157-163 :: ID: 420853
Abstract
BACKGROUND: Acid back-diffusion activates capsaicin-sensitive sensory neurons(CSN), leading to gastric hyperemic response. We examined the role of vanilloid type-1 receptor (VR1)in gastric hyperemic and ulcerogenic responses in rat stomach following exposure to taurocholate (TC).MATERIAL/METHODS: Under urethane anesthesia, a rat stomach was mounted on an ex-vivo chamber, perfusedwith 50 mM HCl, and changes in PD, gastric mucosal blood flow (GMBF), and luminal acid loss were measuredbefore and after exposure to 20 mM TC for 30 min, in presence of omeprazole. Capsazepine was co-appliedwith TC for 30 min to the stomach, while ruthenium red was given i.v. 10 min before TC treatment. RESULTS:TC caused a marked PD reduction, followed by an increase of acid loss and GMBF, resulting in minimaldamage in the mucosa. Chemical ablation of CSN attenuated the GMBF response to TC without affecting PDreduction and acid loss, and resulted in severe lesions, while none of these responses induced by TCwas significantly affected by either capsazepine or ruthenium red. Intragastric capsaicin increased GMBF,and this response was attenuated by both capsazepine and ruthenium red as well as sensory deafferentation.CONCLUSIONS: Both acid back-diffusion and capsaicin increase GMBF mediated by CSN, yet their modes ofaction differ in terms of capsazepine- or ruthenium red-sensitivity. Although the luminal H+ plays amodulator role for the physiological response mediated by CSN in the stomach, it is unlikely that theaction results from the interaction of H+ with the capsazepine- or ruthenium red-sensitive site of VR1.
Keywords: Capsaicin, Diffusion, Gastric Acid, Gastric Mucosa, Hydrogen, Receptors, Drug, Ruthenium Red, Stomach, stomach ulcer, Time Factors
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952