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Accuracy of t-PA, u-PA, PAI-1 and PAI-2 estimation in human bile by ELISA kits.

Zdzisława Kondera-Anasz, Aleksander Michalski, Dariusz Gil, Bożena Gil, Jacek Starzewski, Zbigniew Gonciarz

Med Sci Monit 2000; 6(3): PI616-617

ID: 421299


The aim of this study was to establish the accuracy of determination offibrinolytic proteins in human bile using commercial kits. MATERIAL AND METHOD: Gall bladder bile wasobtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogenactivator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2)in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'.RESULTS: Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2.We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercialkits.

Keywords: Bile, Cholecystectomy, Laparoscopic, Enzyme-Linked Immunosorbent Assay, Humans, Plasminogen Activator Inhibitor 1, Plasminogen Activator Inhibitor 2, Reagent Kits, Diagnostic, Reproducibility of Results, Tissue Plasminogen Activator, Urinary Plasminogen Activator