Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 January 1998

Relationship between LPS E. coli and Intralipid in the influence of peripheral blood neutrophils examined by the NBT reduction tests

Ewa Pawłowska, Gabriel Turowski

Med Sci Monit 1998; 4(1): BR57-63 :: ID: 502827

Abstract

The effect of LPS E. coli in different concentrations: 0.2, 1.0, 2.0, 4.0, 5.0 µg in constant volume of 0.01 ml of KRB and of Intralipid (ILP) in different dosages: 0.5, 1.0, 1.5, 2.0, 3.0 mg in 0.01 ml KRB, and also the mutual influence of LPS E. coli and Intralipid on metabolic activity of neutrophils reduction tests of sodium salt of nitroblue tetrazolium (NBT) were used.Stimulating effect of LPS E. coli was depended on used dosage. Dosages 0.2-1.0 µg of LPS proved to be insufficient for the stimulation of neutrophils. However statistically significant differences, in comparison to control, were ascertained for dosages 2.0-5.0 µg of LPS. Intralipid caused stimulation of neutrophils conditioned by size of used dosage. Obtained results of NBT test, stimulated by different ILP dosages, were highly statistically significant in comparison to control results. In combine use of ILP and LPS, for small dosages of LPS (o, 2-2.0 µg) stimulation was caused by ILP. In the use of 5.0 µg dosage of LPS statistically significant differences, in results of influence of LPS and ILP used together, in comparison to results of effect of LOPS, as well as ILP, when those factors worked separately were ascertained. It may point to stimulating effect of both factors combined on the metabolic activity of neutrophils.he research did not show a function of Intralipid in the neutralization of LPS E. coli. The experiment enable the choice of dosages: 4.0 µg LPS E. coli, and 2.0 mg of Intralipid as optimal for farther research of role of neutrophils in neutralization of endotoxin.

Keywords: Neutrophils, endotoxin (LPS), Intralipid (ILP), NBT reduction test

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750