Combined impact of matrix metalloproteinase-3 and paraoxonase 1 55/192 gene variants on coronary artery disease in Turkish patients
Elif Ozkok, Makbule Aydin, Erhan Babalik, Zeynep Ozbek, Nurhan Ince, Ihsan Kara
Med Sci Monit 2008; 14(10): CR536-542
We investigated the association of matrix metalloproteinase-3 (MMP-3) and paraoxonase 1 (PON1) 55/192 polymorphisms with coronary artery disease (CAD) and the number of diseased vessels in patients with CAD.
Material and Method: One hundred thirty-nine CAD patients and 119 healthy control subjects were included in the study. Genotypes for PON1 55/192 and MMP-3 5A/6A polymorphisms were determined by restriction fragment length polymorphism.
Results: Although distributions of the RR genotype of PON1 192 and the 5A5A genotype of MMP-3 were more frequent in patients, frequencies of the QQ genotype of PON1 192, the MM genotype of PON1 55, and the 6A6A genotype of MMP-3 were significantly lower in patients compared with healthy control subjects. The combined genotypes of RR/LL and/or 5A5A are increased the risk of CAD when compared with subjects who possess neither the MMP-3 5A5A nor the PON1 RR/LL genotype. While the MMP-3 5A/6A genetic variants were not associated with the number of diseased vessels, PON1 55/192 variants were associated with the number of diseased vessels.
Conclusions: The combined PON1 55/192 and MMP-3 5A/6A genetic variants are associated with CAD; PON1 seems to be connected with the number of diseased vessels, and hypertension and hyperlipidemia are related with PON1 192 and MMP-3 in CAD patients.
Keywords: Polymorphism, Genetic, Myocardial Infarction - genetics, Middle Aged, Matrix Metalloproteinase 3 - genetics, Male, Hypertension - genetics, Hyperlipidemias - genetics, Humans, Genetic Predisposition to Disease, Genotype, Female, Diabetes Mellitus - genetics, Turkey, Polymorphism, Restriction Fragment Length, Coronary Artery Disease - pathology, Aryldialkylphosphatase - genetics, Alleles, Aged, Adult