Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

29 January 2009

Osteoactivin bioinformatic analysis: prediction of novel functions, structural features, and modes of action

Abdulhafez A. Selim

Med Sci Monit 2009; 15(2): MT19-33 :: ID: 869538

Abstract

Background
The initial identification of osteoactivin (OA) came from studies using an animal model of osteopetrosis in rats. Several recent studies suggested roles for OA in osteoblast differentiation, fibroblast differentiation, cancer metastasis, and attenuation of muscle, liver, and kidney injury-induced degeneration.
Material and Method
Several bioinformatic tools were utilized, including ArrayExpress, Pfam, SMART, Prosite, ELM, ProFun, PFP, Consensus Secondary Structure Prediction server, and Geno3D.
Results
1) Novel OA functions and biological roles were predicted, including roles in cell envelope formation, enzyme activities, immune response activities, negative regulation of B-cell activation, antigen processing, heme catabolism, endothelial cell differentiation, establishment of protein localization, melanin biosynthesis from tyrosine, regulation of blood pressure, response to light, and lung development. 2) Novel OA functional motifs were predicted, including N-Arg dibasic convertase cleavage site, NEC1/NEC2 cleavage site, peptide C-terminal amidation, glycosaminoglycan attachment site, and generic motif for N-glycosylation, and substrate recognition sites that interact with different cytosolic proteins, including cyclin, MAPK, Class III PDZ domains, GSK3, phosphorylase kinase, tyrosine-based sorting signal, and internalization signal. 3) OA's secondary and tertiary structural models were predicted.
Conclusions
bioinformatic structural analysis predicted that OA has three major structural domains: two helical structures on its termini and beta sheets as a sandwich-like structure in the middle. Several functional motifs have been predicted suggesting different modes for OA functions (receptor, ligand, and enzyme). Combined data from OA's expression array and function prediction data suggest that OA might play a role in several pathologic conditions, including hypertension, diabetes, and immune system disorders.

Keywords: Software, Protein Structure, Secondary, Oligonucleotide Array Sequence Analysis, Molecular Sequence Data, Models, Molecular, Membrane Glycoproteins - metabolism, Internet, Gene Expression Regulation, Gene Expression Profiling, Protein Structure, Tertiary, Computational Biology - methods, Amino Acid Sequence, Amino Acid Motifs

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750