03 June 2004
Loss of heterozygosity on chromosome arm 13q in larynx cancer patients: analysis of tumor, margin and clinically unchanged mucosa
Katarzyna Szukała, Jürgen Brieger, Katharina Bruch, Wiesława Biczysko, Małgorzata Wierzbicka, Witold Szyfter, Krzysztof SzyfterMed Sci Monit 2004; 10(6): CR233-240 :: ID: 11674
Abstract
Background:Loss of heterozygosity, frequently observed during the development of many tumor types, also occurs in larynx cancer. This disease has a very complex genetic background, with numerous alterations involving oncogenes and tumor suppressor genes. The upper parts of the respiratory-digestive tract are exposed to many carcinogens, which can result in the appearance of multiple tumors or primary tumor relapses. The examination of normal-appearing tissues makes it possible to recognize genetic events occurring at early stages.Material/Methods: 65 larynx cancer patients were examined for loss of heterozygosity (LOH) on the 13q chromosomal arm, with the application of three microsatellite markers. The material from each patient consisted of blood, tumor, safe margin, and 1–2 clinically unchanged mucosal samples.Results: High frequencies of LOH in tumor tissues (49–64%) were observed in both studied chromosomal regions (13q14 and 13q34). The frequency of LOH in safe margin ranged from 12 to 31%. In normal-appearing mucosa, LOH was observed less frequently: 6–26% of informative cases.Conclusions: The data obtained from this investigation suggest that losses in the region of the 13q arm occur frequently during larynx cancer development. Moreover, they were observed not only in tumor tissues, but also in clinically unchanged mucosa. This could be a highly reliable predictor of the occurrence of relapse or second primary tumor in this anatomical site.
Keywords: Base Sequence, Chromosomes, Human, Pair 13 - genetics, Follow-Up Studies, Laryngeal Neoplasms - genetics, Laryngeal Neoplasms - pathology, Loss of Heterozygosity - genetics, Mucous Membrane - metabolism, Mucous Membrane - pathology, Base Sequence, Chromosomes, Human, Pair 13 - genetics, Follow-Up Studies, Laryngeal Neoplasms - pathology, Loss of Heterozygosity - genetics, Mucous Membrane - pathology
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952