01 February 2005
Evidence of the glyoxylate cycle in the liver of newborn rats
Igor G. Morgunov, Maria N. Kondrashova, Svetlana V. Kamzolova, Alexander P. Sokolov, Nadezhda I. Fedotcheva, Tatiana V. FinogenovaMed Sci Monit 2005; 11(2): BR57-60 :: ID: 14143
Abstract
Background:It is generally accepted that the glyoxylate cycle exists in microorganisms and higher plants but absent in higher animals. The hypothesis of the glyoxylate cycle in the tissues of higher animals with a high level of physiological activity was first proposed by Kondrashova and Rodionova in 1971. The goal of this work was to verify this in newborn rats, which possess a 2.5-fold higher physiological activity and oxygen consumption rate than adult rats.Material/Methods:Newborn (7-day-old) and adult rats were used for this experiment. The activities of the key enzymes of the glyoxylate cycle (isocitrate lyase and malate synthase) were measured by HPLC and spectroscopic methods.Results:The activities of isocitrate lyase and malate synthase were found in the liver homogenates prepared from newborn rats, but not from adult rats. The activities of the enzymes common to both the Krebs cycle and the glyoxylate cycle (citrate synthase, aconitase, and malate dehydrogenase) were 20–40% higher in newborn than in adult rats.Conclusions:These data indicate the existence of the glyoxylate cycle in animal tissues.
Keywords: Aconitate Hydratase - metabolism, Citrate (si)-Synthase - metabolism, Glyoxylates - metabolism, Isocitrate Lyase - metabolism, Malate Dehydrogenase - metabolism, Malate Synthase - metabolism, Aconitate Hydratase - metabolism, Aging - physiology, Animals, Newborn, Chromatography, High Pressure Liquid, Citrate (si)-Synthase - metabolism, Citric Acid Cycle, Glyoxylates - metabolism, Isocitrate Lyase - metabolism, Liver - metabolism, Malate Dehydrogenase - metabolism, Malate Synthase - metabolism, Rats, Wistar
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952