01 January 2002
Intracerebroventricular administration of bacterial lipopolysaccharide prevents the development of acute experimental pancreatitis in the rat.
Anna Leja, Ryszard Sendur, Katarzyna Nawrot, Joanna Bonior, Jolanta Jaworek, Jerzy Stachura, Wiesław Pawlik, Stanisław KonturekMed Sci Monit 2002; 8(4): BR136-143 :: ID: 420879
Abstract
BACKGROUND: Lipopolysaccharides (LPS) are responsible for septic shockbut low doses of LPS reduce pancreatic damage produced by caerulein-induced pancreatitis (CIP) in rats.Leptin, produced by adipocytes attenuates the severity of CIP. The aim of this study was to evaluatethe effect of intracerebroventricular (i.c.v.) administration of LPS on CIP and plasma leptin level andto investigate the involvement of sensory nerves (SN) in the effects of LPS on CIP. MATERIAL/METHODS:CIP was produced by subcutaneous (s.c.) infusion of caerulein (25 Kg/kg) to conscious rats. SN were deactivatedwith capsaicin (100 mg/kg s.c.). LPS (0.2, 2, or 20 Kg/rat) were applied to the right cerebral ventricle30 min prior to CIP. RESULTS: CIP was manifested by an increase in plasma levels of amylase, lipase,leptin and an anti-inflammatory interleukin 10 (IL-10), (by 400%, 1000%, 700% and 50%, respectively),confirmed by histological examination and accompanied by 40% reduction in pancreatic blood flow. Pretreatmentof CIP rats with i.c.v. LPS resulted in significant reduction of CIP accompanied by dose-dependent increasein plasma levels of leptin and IL-10. Deactivation of SN, which by itself failed to affect CIP, completelyreversed the beneficial effects of i.c.v. administration of LPS on CIP and reduced plasma leptin andIL-10 concentrations. CONCLUSIONS: Pretreatment with LPS given i.c.v. prevents the development of caerulein-inducedpancreatitis through the activation of SN and though the release of leptin.
Keywords: Adipocytes, Afferent Pathways, amylases, Caerulein, Capsaicin, Comparative Study, Escherichia coli, Injections, Intraventricular, Interleukin-10, Leptin, Lipase, Neurons, Afferent, Rats, Wistar, Sympathectomy, Chemical
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
18 Mar 2024 : Clinical Research
Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative StudyMed Sci Monit In Press; DOI: 10.12659/MSM.944136
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
Most Viewed Current Articles
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952