01 November 2007
Distribution of TEM and SHV Beta-lactamase genes among Klebsiella pneumoniae strains isolated from patients in Tehran
Fereshteh Shahcheraghi, Haleh Moezi, Mohammad Mehdi FeizabadiMed Sci Monit 2007; 13(11): BR247-250 :: ID: 512825
Abstract
Background: There are numerous types of extended spectrum beta-lactamases (ESBLs), of which TEM and SHV are predominant among Klebsiella pneumoniae strains. Nosocomial infections with strains of K. pneumoniae are common in healthcare centers in Iran. However, no information is available on the prevalence of different phenotypes of ESBL strains in Tehran hospitals.
Material/Methods: To determine the resistance of K. pneumoniae to beta-lactam antibiotics in Tehran hospitals, 145 isolates were tested using the disk diffusion method. The MICs for ceftazidime were also determined using microbroth dilution assay. Isolates showing MIC ≥4 for ceftazidime were subjected to PCR to target the bla[sub]SHV[/sub] and bla[sub]TEM[/sub] genes and screened for ESBL production by the phenotypic confirmatory method.
Results: All strains were susceptible to imipenem. Resistance to ceftazidime and cefotaxime were 31% and 32%, respectively. Fifty-six isolates showed MIC ≥4 µg/ml for ceftazidime, of which 50 were positive for ESBL in the phenotypic confirmatory test. The prevalence of bla[sub]SHV[/sub] and bla[sub]TEM[/sub] among these isolates was 69.6% (n=39) and 32.1% (n=18), respectively. Resistance to ciprofloxacin was found among 32% of the ESBL strains.
Conclusions: SHV is the dominant enzyme among the ESBL-producing strains of Klebsiella pneumoniae in Iran. The high rate of co-resistance to ceftazidime and ciprofloxacin is a matter of concern and treatment requires further attention to the results of susceptibility.
Keywords: Base Sequence, beta-Lactamases - genetics, Polymerase Chain Reaction, Microbial Sensitivity Tests, Klebsiella pneumoniae - isolation & purification, Klebsiella Infections - microbiology, Iran, Genes, Bacterial, Drug Resistance, Bacterial, DNA, Bacterial - genetics, DNA Primers - genetics, Cross Infection - microbiology, Ceftazidime - pharmacology, Cefotaxime - pharmacology, Cefotaxime - pharmacology, beta-Lactamases - genetics, Polymerase Chain Reaction, Microbial Sensitivity Tests, Klebsiella pneumoniae - isolation & purification, Klebsiella Infections - microbiology, Iran, Genes, Bacterial, Drug Resistance, Bacterial, DNA, Bacterial - genetics, DNA Primers - genetics, Cross Infection - microbiology, Ceftazidime - pharmacology, Base Sequence
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
18 Mar 2024 : Clinical Research
Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative StudyMed Sci Monit In Press; DOI: 10.12659/MSM.944136
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
Most Viewed Current Articles
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952