01 July 2008
Comparison of N-epsilon-(Carboxymethyl)Lysine levels and percentage of eosinophils in induced sputum for assessment of small airway involvements in asthma
Shigenori Kyoh, Hiroshi Kanazawa, Yoshihiro Tochino, Toyoki Kodama, Kazuhisa Asai, Kazuto HirataMed Sci Monit 2008; 14(7): CR375-380 :: ID: 863667
Abstract
Background: N-epsilon-(Carboxymethyl)Lysine (CML), a major advanced glycation end product, is expressed in the lower respiratory tract. In this study, we compared the validity of measuring CML levels and percentage of eosinophils in induced sputum for assessment of airway functions, and evaluated the clinical implications of sputum CML levels in the asthmatic airways.
Material/Methods: We examined CML levels and percentage of eosinophils in induced sputum from 37 asthmatic patients and 15 normal controls, and evaluated the relationships between these parameters and clinical profiles of asthmatic patients.
Results: The percentage of eosinophils in induced sputum was significantly higher in asthmatic patients than in normal controls. Similarly, CML levels were also significantly higher in asthmatic patients than in normal controls (median (range): asthmatic patients 29.3 (18.4–64.7) µg/mL; normal controls 25.8 (14.0–47.0) µg/mL, p=0.02). However, there was no significant correlation between CML level and percentage of eosinophils. In asthmatic patients, percentage of eosinophils was significantly correlated with FEV1/FVC and degree of airway hyperreactivity to methacholine. However, CML level was correlated with FEV1/FVC, but not with methacholine reactivity. In contrast, CML level, but not percentage of eosinophils, was significantly correlated with degree of small airways dysfunction.
Conclusions: Our novel, non-invasive technique of measurement of CML levels in induced sputum may prove to be important not only in the evaluation of small airway involvements, but also in helping us move toward a better understanding of the roles of the small airways in the pathogenesis of asthma.
Keywords: Asthma, advanced glycation end products, small airways, induced sputum, Asthma - pathology, Case-Control Studies, Eosinophils - pathology, Forced Expiratory Volume, Lysine - analysis, Methacholine Chloride - therapeutic use, Respiratory System - pathology, Sputum - chemistry
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
18 Mar 2024 : Clinical Research
Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative StudyMed Sci Monit In Press; DOI: 10.12659/MSM.944136
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
Most Viewed Current Articles
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952