Figure 4 Circ_0005556 was bound by miR-767-5p. (A) Circ_0005556 levels were measured in GC cell lines (MGC-803, SGC-7901, AGS, HGC-27, and BGC-823), (* P<0.05, ** P<0.01, *** P<0.001 vs. GES-1 group). (B) Target miRNAs of circ_0005556 were predicted by bioinformatics method, and the relative fold change of these target miRNAs in BGC-823 was detected. The physical interplay between circ_0005556 and miR-767-5p was validated by (C) a dual-luciferase reporter experiment and (D) a circRNA pulldown assay in BGC-823 cells (* P<0.05, *** P<0.001). (E) The relative expression level of miR-767-5p was investigated by qRT-PCR in 118 pairs of GC and normal tissue samples (*** P<0.001). (F) Correlation analysis between circ_0005556 level and miR-767-5p level in GC tissue specimens (P=0.0104).