Figure 2 The effect of berberine on the clinical indexes of insomnia in rats. (A) Berberine and diazepam restored the body weight of insomnia rats. (B) Berberine and diazepam reduced the sleep latency of insomnia rats and prolonged the sleep duration in pentobarbital sodium test. (C) In the water maze experiment, the time of finding the platform in insomniac rats treated with berberine and diazepam decreased, and the number of times of crossing the platform increased. (D) The expression of ANP, BNP, and ET-1 in insomnia rats after berberine and diazepam treatment were decreased. * P<0.05, ** P<0.01, *** P<0.001 versus model group; # P<0.05, ## P<0.01 versus diazepam group. ANP – atrial natriuretic peptide; BNP – B-type natriuretic peptide; ET-1 – endothelin-1.