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29 July 2020: Lab/In Vitro Research

Long Non-Coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)Relieves Sepsis-Induced Kidney Injury and Lipopolysaccharide (LPS)-Induced Inflammation in HK-2 Cells

Cheng Gao 1ABF* , Xiequn Zou 1BCF* , Huan Chen 2CD , Rong Shang 3BD , Bing Wang 1BD*

DOI: 10.12659/MSM.921906

Med Sci Monit 2020; 26:e921906

Figure 2 Effects of NEAT1 silencing on proliferation, apoptosis, and inflammation of LPS-treated HK-2 cells. (A) NEAT1 level was assessed in HK-2 cells treated with different concentrations LPS (0, 0.1, 1.0, 10, and 20 mg/L) for 24 h by RT-qPCR assay. (B) NEAT1 level was detected in HK-2 cells stimulated with 1.0 mg/L LPS at various time points (0, 6, 12, 18, and 24 h) by RT-qPCR assay. (C–F) HK-2 cells were separated into 4 groups: control, LPS, LPS+si-NC, and LPS+si-NEAT1 groups. (C) The relative expression level of NEAT1 was quantified with RT-qPCR analysis in HK-2 cells. (D) The growth curves of HK-2 cells were drawn using MTT assay. (E) Flow cytometry was used to measure the apoptosis rate of HK-2 cells. (F) The protein expression levels of TNF-α, IL-6, and IL-1β were assessed with Western blot analysis. * P<0.05.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750