Figure 3 Let-7b-5p was a direct target of NEAT1. (A) The complementary sequence between let-7b-5p and NEAT1 along with mutant sites were shown. (B) The luciferase activity was analyzed by dual-luciferase reporter assay. (C) RT-qPCR assay was used to test the expression level of let-7b-5p in kidney tissues in sepsis patients and healthy kidney tissues. (D, E) Let-7b-5p expression was analyzed by RT-qPCR assay in HK-2 cells. (F) RT-qPCR analysis was used to analyze let-7b-5p expression in HK-2 cells treated with LPS+si-NC, LPS+si-NEAT1, LPS+pcDNA, or LPS+NEAT1. * P<0.05.