29 July 2020: Lab/In Vitro Research
Long Non-Coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)Relieves Sepsis-Induced Kidney Injury and Lipopolysaccharide (LPS)-Induced Inflammation in HK-2 Cells
Cheng Gao 1ABF* , Xiequn Zou 1BCF* , Huan Chen 2CD , Rong Shang 3BD , Bing Wang 1BD*DOI: 10.12659/MSM.921906
Med Sci Monit 2020; 26:e921906
Figure 3 Let-7b-5p was a direct target of NEAT1. (A) The complementary sequence between let-7b-5p and NEAT1 along with mutant sites were shown. (B) The luciferase activity was analyzed by dual-luciferase reporter assay. (C) RT-qPCR assay was used to test the expression level of let-7b-5p in kidney tissues in sepsis patients and healthy kidney tissues. (D, E) Let-7b-5p expression was analyzed by RT-qPCR assay in HK-2 cells. (F) RT-qPCR analysis was used to analyze let-7b-5p expression in HK-2 cells treated with LPS+si-NC, LPS+si-NEAT1, LPS+pcDNA, or LPS+NEAT1. * P<0.05.