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29 July 2020: Lab/In Vitro Research

Long Non-Coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)Relieves Sepsis-Induced Kidney Injury and Lipopolysaccharide (LPS)-Induced Inflammation in HK-2 Cells

Cheng Gao 1ABF* , Xiequn Zou 1BCF* , Huan Chen 2CD , Rong Shang 3BD , Bing Wang 1BD*

DOI: 10.12659/MSM.921906

Med Sci Monit 2020; 26:e921906

Figure 4 Knockdown of NEAT1-mediated effects on proliferation, apoptosis, and inflammation in LPS-induced HK-2 cells was abolished by silencing of let-7b-5p. (A) Relationship between the expression levels of NEAT1 and let-7b-5p was analyzed by Pearson’s correlation analysis. (B–E) HK-2 cells were exposed to LPS+si-NC, LPS+si-NEAT1, LPS+si-NEAT1+anti-let-NC, or LPS+si-NEAT1+anti-let-7b-5p. (B) RT-qPCR was enforced to measure expression level of let-7b-5p in HK-2 cells. (C) MTT assay was performed for examining the cell viability of HK-2 cells. (D) The apoptosis rate of HK-2 cells was assessed by performing flow cytometry assay. (E) The protein expressions of TNF-α, IL-6, and IL-1β in HK-2 cells were examined by Western blot assay. * P<0.05.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750