Figure 5 Let-7b-5p negatively regulated TRAF6 expression. (A) Binding region between let-7b-5p and TRAF6 as well as mutated nucleotides ofTRAF6 3′UTR were shown. (B) Dual-luciferase reporter assay was used to show the luciferase activity of TRAF6 3′UTR-WT and TRAF6 3′UTR-MUT in HK-2 cells. (C, D) The expression levels of TRAF6 in kidney tissues from sepsis patients and healthy kidney tissues were evaluated by RT-qPCR and Western blot assay, respectively. (E–H) LPS elevated TRAF6 expression in HK-2 cells. (I, J) Transfection efficiency of let-7b-5p and anti-let-7b-5p was checked by RT-qPCR assay, with let-NC and anti-let-NC as controls. (K, L) The expression level of TRAF6 was analyzed with RT-qPCR and Western blot assay in HK-2 cells treated with LPS+let-NC, LPS+let-7b-5p, LPS+anti-let-NC, or LPS+anti-let-7b-5p. * P<0.05.