Figure 6 Overexpression of TRAF6 reversed let-7b-5p-induced the effect on proliferation, apoptosis, and inflammation in LPS-induced HK-2 cells. (A–F) HK-2 cells were treated with LPS+let-NC, LPS+let-7b-5p, LPS+let-7b-5p+pcDNA, or LPS+let-7b-5p+TRAF6. (A, B) The level of TRAF6 in HK-2 cells was measured by using RT-qPCR and Western blot assays. (C) Pearson’s correlation analysis revealed a negative correlation between the levels of TRAF6 mRNA and let-7b-5p. (D) Cell proliferation capability was examined in HK-2 with MTT analysis. (E) The apoptosis of HK-2 cells was performed using flow cytometry assay. (F) The expression levels of TNF-α, IL-6, and IL-1β were shown in HK-2 cells with Western blot analysis. * P<0.05.