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29 July 2020: Lab/In Vitro Research

Long Non-Coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)Relieves Sepsis-Induced Kidney Injury and Lipopolysaccharide (LPS)-Induced Inflammation in HK-2 Cells

Cheng Gao 1ABF* , Xiequn Zou 1BCF* , Huan Chen 2CD , Rong Shang 3BD , Bing Wang 1BD*

DOI: 10.12659/MSM.921906

Med Sci Monit 2020; 26:e921906

Figure 6 Overexpression of TRAF6 reversed let-7b-5p-induced the effect on proliferation, apoptosis, and inflammation in LPS-induced HK-2 cells. (A–F) HK-2 cells were treated with LPS+let-NC, LPS+let-7b-5p, LPS+let-7b-5p+pcDNA, or LPS+let-7b-5p+TRAF6. (A, B) The level of TRAF6 in HK-2 cells was measured by using RT-qPCR and Western blot assays. (C) Pearson’s correlation analysis revealed a negative correlation between the levels of TRAF6 mRNA and let-7b-5p. (D) Cell proliferation capability was examined in HK-2 with MTT analysis. (E) The apoptosis of HK-2 cells was performed using flow cytometry assay. (F) The expression levels of TNF-α, IL-6, and IL-1β were shown in HK-2 cells with Western blot analysis. * P<0.05.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750