24 August 2020: Animal Study
Qi-Xian Decoction Upregulated E-cadherin Expression in Human Lung Epithelial Cells and Ovalbumin-Challenged Mice by Inhibiting Reactive Oxygen Species-Mediated Extracellular-Signal-Regulated Kinase (ERK) Activation
Lingling Tang 1BCDG* , Linyun Zhu 1BCD* , Wei Zhang 2CDF* , Xiaoyan Yang 1DE , Qingge Chen 1DG , Ziyu Meng 1D , Jinjin Liu 1E , Yipeng Sun 1F , Junsheng Hu 1F , Zhenhua Ni 3AEG* , Xiongbiao Wang 1AEG*DOI: 10.12659/MSM.922003
Med Sci Monit 2020; 26:e922003
Figure 5 QXT attenuated ERK activation in OVA-challenged mice and human lung epithelial cells. (A, B) ERK1/2 phosphorylation levels were examined by Western blot (n=8). (C, D) 16HBE cells were treated with H2O2 (40 μM) plus the water or ethanol extract from QXT (80 μg/mL) for 48 h. The protein levels of phospho-ERK and ERK were detected by Western blotting (n=3). (B, D) Phospho-ERK protein concentrations were quantified by using Image J software and normalized to β-actin. Data are shown as the mean±standard, * P<0.05, ** P<0.01.