Figure 2 Rab7 promoted the GC cells proliferation ability and changed the cell cycle. (A) Western blot analysis confirmed that Rab7 was overexpressed in AGS cells and was knocked down in MCG803 and HGC-27 cells. μ-actin acted as loading control. (B–D) Proliferation rate of GC cells after depleting or strengthening Rab7 expression, as determined by the CCK8 assay. The results revealed that knockdown of Rab7 suppressed MCG803 and HGC-27 cells proliferation and overexpression of Rab7-promoted AGS cells proliferation. The data represent the mean±SD of 3 independent experiments, * P<0.05, by 2-tailed t test). (E, F) the cycle changes of GC cells were detected by flow cytometry (data represent the mean±SD of 3 independent experiments, * P<0.05, by 2-tailed t test).