Figure 3 Rab7 regulated GC cells migration and invasion potential. (A, B) Migration ability of GC cells was measured by transwell chamber migration assay. Scale bar, 50 μm. The results showed that Rab7 knockdown results in suppressed MCG803 and HGC-27 cells migration ability, while the overexpression of Rab7 promoted the AGS cells migration ability. Data represent the mean±SD of 3 independent experiments, * P<0.05, by 2-tailed t test). (C, D) Invasion ability of gastric cancer (GC) cells was measured by transwell chamber invasion assay. Scale bar, 50 μm. The results showed that Rab7 knockdown results in suppressed MCG803 andHGC-27cells invasion ability, while the overexpression of Rab7 promoted the invasion ability of AGS cells. Statistical analysis revealed that, compared with p-super groups, the psh-Rab7 groups had significantly fewer invading and migrating cells, and, compared with p-CDH groups, the p-Rab7 groups had significantly more invading and migrating cells. Data represent the mean±SD of 3 independent experiments. * P<0.05, n=5 random fields, by 2-tailed t test).