05 August 2020>: Clinical Research
Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p
Ke Hu BCD , Xianhao Liu BCD , Yi Li ABC , Qinyang Li DEF , Yijun Xu DEF , Wei Zeng DEF , Guocheng Zhong CDEF , Changhua Yu CDE*DOI: 10.12659/MSM.922253
Med Sci Monit 2020; 26:e922253
Figure 2 Circ_UBE2D2 deletion mitigates tamoxifen resistance in tamoxifen-resistant breast cancer cells. (A, B) qRT-PCR analysis on the expression of circ_UBE2D2 in tamoxifen-resistant and tamoxifen-sensitive breast cancer patients, as well as in parent and resistant breast cancer cells was conducted. MCF-7/TAM-R and T47D/TAM-R cells were transfected with si-circ_UBE2D2 or si-NC. (C) The expression of circ_UBE2D2 in MCF-7/TAM-R and T47D/TAM-R cells was examined using qRT-PCR to analyze the interference efficiency. (D) The viability and IC50 values of MCF-7/TAM-R and T47D/TAM-R cells treated with different concentrations of tamoxifen were detected by CCK-8 assay. (E, F) The migration and invasion analysis of parental and resistant cells treated with 20 μM tamoxifen was performed using Transwell assay. (G) The levels of ERα, vimentin, and E-cad in parent and resistant cells treated with 20 μM tamoxifen were detected using western blot. * P<0.05, ** P<0.01. qRT-PCR – real-time polymerase chain reaction; CCK-8 – Cell Counting Kit-8; ERα – estrogen receptor alpha; E-cad – E-cadherin.