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05 August 2020: Clinical Research

Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p

Ke Hu BCD , Xianhao Liu BCD , Yi Li ABC , Qinyang Li DEF , Yijun Xu DEF , Wei Zeng DEF , Guocheng Zhong CDEF , Changhua Yu CDE*

DOI: 10.12659/MSM.922253

Med Sci Monit 2020; 26:e922253

Figure 3 Upregulation of circ_UBE2D2 in exosomes from tamoxifen-resistant breast cancer cells. Cell exosomes were extracted from tamoxifen-resistant and parental breast cancer cells. (A) The image of exosomes was captured by TEM, and the expression levels of exosomal markers CD9 and CD63 were measured by western blot. (B) Circ_UBE2D2 expression in MCF-7/TAM-R-exo and MCF-7/Par-exo was detected by qRT-PCR. (C) qRT-PCR analysis of circ_UBE2D2 was conducted in the MCF-7/TAM-R-exo co-cultured parental cells untreated with or treated with cytochalasin D. * P<0.05, ** P<0.01. TEM – transmission electron microscopy; qRT-PCR – real-time polymerase chain reaction.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750