05 August 2020: Clinical Research
Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p
Ke Hu BCD , Xianhao Liu BCD , Yi Li ABC , Qinyang Li DEF , Yijun Xu DEF , Wei Zeng DEF , Guocheng Zhong CDEF , Changhua Yu CDE*DOI: 10.12659/MSM.922253
Med Sci Monit 2020; 26:e922253
Figure 4 Intercellular transfer of circ_UBE2D2 by exosomes enhances tamoxifen resistance in breast cancer cells in vitro. (A) Cell viability and IC50 values were detected using CCK-8 assay in MCF-7/Par and T47D/Par cells treated with tamoxifen at different concentrations. (B, C) The migration and invasion of parental cells treated with 10 μM tamoxifen were examined using Transwell assay. (D) Levels analysis of ERα, vimentin, and E-cad in parental cells treated with 10 μM tamoxifen were carried out using western blot. * P<0.05, ** P<0.01. ERα – estrogen receptor alpha; E-cad – E-cadherin.