05 August 2020: Clinical Research
Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p
Ke Hu BCD , Xianhao Liu BCD , Yi Li ABC , Qinyang Li DEF , Yijun Xu DEF , Wei Zeng DEF , Guocheng Zhong CDEF , Changhua Yu CDE*DOI: 10.12659/MSM.922253
Med Sci Monit 2020; 26:e922253
Figure 5 MiR-200a-3p is target of circ_UBE2D2. (A, B) qRT-PCR analysis of the expression of miR-200a-3p in tamoxifen-resistant and -sensitive tissues, as well as in parent and resistant cells in breast cancer was conducted. (C) The correlation between miR-200a-3p and circ_UBE2D2 was analyzed using Pearson correlation analysis. (D) The putative binding site between miR-200a-3p and circ_UBE2D2 was presented. (D, E) The interaction between miR-200a-3p and circ_UBE2D2 was confirmed using the dual-luciferase reporter assay and RIP assay. * P<0.05, ** P<0.01. qRT-PCR – real-time polymerase chain reaction; RIP – RNA immunoprecipitation.