Figure 6 Exosomes mediated transfer of circ_UBE2D2 increases tamoxifen resistance in breast cancer cells by binding to miR-200a-3p. Parental cells co-cultured with MCF-7/TAM-R-exo were transfected with miR-NC or miR-200a-3p mimic. (A, B) The viability and IC50 values in MCF-7/Par and T47D/Par cells treated with different concentrations of tamoxifen were detected using CCK-8 assay. (C, D) The migrated and invaded parental cells treated with 10 μM tamoxifen were evaluated by Transwell assay. (E) Western blot was used to measure the levels of ERα, vimentin, and E-cad in parental cells treated with 10 μM tamoxifen. * P<0.05, ** P<0.01. CCK-8 – Cell Counting Kit-8; ERα – estrogen receptor alpha; E-cad – E-cadherin.