10 October 2020 : Clinical Research
miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages
Junfeng Heng1CDEF, Dingye Wu2BCD, Shiqi Lu1CDE*, Yiming Zhao2ABCDDOI: 10.12659/MSM.922479
Med Sci Monit 2020; 26:e922479
Figure 2 miR-106a expression is increased and anoctamin 1 (ANO1) expression is decreased in lipopolysaccharide (LPS)-treated RAW264.7 cells. (A, B) Messenger ribonucleic acid levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were measured in RAW264.7 cells after treatment with LPS or not by quantitative real-time polymerase chain reaction. (C, D) Secretion levels of IL-6 and TNF-α were detected after treatment with LPS or not by enzyme-linked immunosorbent assay. (E, F) Abundances of miR-106a and ANO1 protein were detected after treatment with LPS or not. * P<0.05.