Figure 3 Overexpression of anoctamin 1 (ANO1) inhibits lipopolysaccharide (LPS)-induced inflammatory response in RAW264.7 cells. (A) Protein expression of ANO1 was detected in LPS-treated RAW264.7 cells with transfection of pcDNA, ANO1 overexpression vector, short interfering (si)-NC, or si-ANO1 by western blot. (B, C) Messenger ribonucleic acid levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were examined in LPS-treated RAW264.7 cells with transfection of pcDNA, ANO1 overexpression vector, si-NC, or si-ANO1 by quantitative real-time polymerase chain reaction. (D, E) Secretion levels of IL-6 and TNF-α were detected in cells transfected with pcDNA, ANO1 overexpression vector, si-NC, or si-ANO1 by enzyme-linked immunosorbent assay. * P<0.05.