10 October 2020 : Clinical Research
miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages
Junfeng Heng1CDEF, Dingye Wu2BCD, Shiqi Lu1CDE*, Yiming Zhao2ABCDDOI: 10.12659/MSM.922479
Med Sci Monit 2020; 26:e922479
Figure 4 Anoctamin 1 (ANO1) is a target of miR-106a in RAW264.7 cells. (A) Binding sequence of miR-106a and ANO1 was searched via DIANA Tools – microT-CDS. (B, C) Luciferase activity was measured in RAW264.7 cells cotransfected with ANO1 3′-untranscribed region wild-type or mutant and miR-NC, miR-106a mimic, in-miR-NC, or in-miR-106a. (D, E) Protein level of ANO1 was detected in RAW264.7 cells transfected with miR-NC, miR-106a mimic, in-miR-NC, or in-miR-106a. * P<0.05.