Figure 5 Overexpression of miR-106a attenuates anoctamin 1 (ANO1)-mediated inflammatory inhibition in lipopolysaccharide (LPS)-treated RAW264.7 cells. (A, B) Messenger ribonucleic acid (mRNA) and protein levels of ANO1 were measured in cells with transfection of pcDNA, ANO1 overexpression vector, ANO1 overexpression vector+miR-NC, or miR-106a mimic. (C, D) mRNA levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) were measured in LPS-treated RAW264.7 cells transfected with pcDNA, ANO1 overexpression vector, ANO1 overexpression vector+miR-NC, or miR-106a mimic via quantitative real-time polymerase chain reaction. (E, F) Secretion levels of IL-6 and TNF-α were detected in cells transfected with pcDNA, ANO1 overexpression vector, ANO1 overexpression vector+miR-NC, or miR-106a mimic by enzyme-linked immunosorbent assay. * P<0.05.