25 September 2020>: Lab/In Vitro Research
Long Non-Coding RNA Paternally Expressed Imprinted Gene 10 (PEG10) Elevates Diffuse Large B-Cell Lymphoma Progression by Regulating Kinesin Family Member 2A (KIF2A) via Targeting MiR-101-3p
Jin Zhao 1BCF* , Liping Su 1BCD* , Jianjun Jiang 2ACE*DOI: 10.12659/MSM.922810
Med Sci Monit 2020; 26:e922810
Figure 3 KIF2A deletion suppresses cell growth, metastasis and elevates cell apoptosis in DLBCL. (A) KIF2A level was detected by RT-qPCR. (B) The protein level of KIF2A was measured by western blot. (C) MTT assay for cell proliferation. (D) Flow cytometry for cell apoptosis. (E) Western blot analysis for the determination of cyclinD1, Bcl-2, caspase-3 and Bax level. (F, G) Transwell assays for cell migration and invasion. * P<0.05. KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.