Figure 6 PEG10 regulates KIF2A level by sponging miR-101-3p. (A) MiRcode showed the putative binding sites between PEG10 and miR-101-3p, while starBase2.0 predicted that the KIF2A mRNA 3′UTR sequence contained the binding sites of miR-101-3p. (B) The luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, WT-PEG10, and MUT-PEG10 was detected by dual-luciferase reporter assay. (C) RT-qPCR was used to examine miR-101-3p level in DLBCL cells transfected with pcDNA, PEG10, si-NC, and si-PEG10. (D) Detection of the luciferase activity in DLBCL cells co-transfected with miR-NC, miR-101-3p, KIF2A-3′UTR-WT, and KIF2A-3′UTR-MUT by dual-luciferase reporter assay. (E) The protein KIF2A level was detected by western blot analysis in DLBCL cells transfected with miR-NC, miR-101-3p, miR-101-3p+pcDNA or miR-101-3p+PEG10. * P<0.05. PEG10 – paternally expressed imprinted gene 10; KIF2A – kinesin family member 2A; DLBCL – diffuse large B-cell lymphoma; RT-qPCR – real-time quantitative polymerase chain reaction.