13 September 2020: Clinical Research
Celastrol Inhibits Migration and Invasion of Triple-Negative Breast Cancer Cells by Suppressing Interleukin-6 via Downregulating Nuclear Factor-κB (NF-κB)
Fei Yan BCDE , Zihong Wu BCD , Zihui Li ABCD , Li Liu BCDE*DOI: 10.12659/MSM.922814
Med Sci Monit 2020; 26:e922814
Figure 4 The effect of celastrol and IL-6 on migration and invasion of TNBC cells. The levels of IL-6 mRNA (A) and protein (B) in cells and in cell medium (C) were measured after exposure to various doses of celastrol for 24 h by qRT-PCR, Western blot, or ELISA, respectively. (D–F) IL-6 expression was measured in MDA-MB-231 and MDA-MB-468 cells with transfection of oe-IL-6 or vector. (G, H) Cell migration and invasion were examined in MDA-MB-231 and MDA-MB-468 cells with transfection of oe-IL-6 or vector after treatment with celastrol. * P<0.05 vs. the corresponding control group (0 μM in A–C; vector group in D–H; celastrol+vector group in G, H).