10 August 2020>: Lab/In Vitro Research
Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells
Xianhao Su ACF* , Liang Chen AD* , Shanliang Han B , Gengming Niu F , Jun Ren C , Chongwei Ke AG*DOI: 10.12659/MSM.922935
Med Sci Monit 2020; 26:e922935
Figure 10 Images of hematoxylin and eosin staining of scaffold. Silk fibroin (SF)/chitosan (Cs) (1: 1) scaffold without cells (A1), SF/Cs/alginate (Alg) (1: 1: 1) scaffold without cells (B1), SF/Cs (1: 1) scaffold with cells on day 3 (A2), SF/Cs/Alg (1: 1: 1) scaffold with cells on day 3 (B2), SF/Cs (1: 1) scaffold with cells on day 7 (A3), SF/Cs/Alg (1: 1: 1) scaffold with cells on day 7 (B3). Spheroids formed in the SF/Cs (1: 1) scaffold are observed under oil lens of Leica DM2500 (spheroid 1). Spheroids formed in the SF/Cs/Alg (1: 1: 1) scaffold are observed under oil lens of Leica DM2500 (spheroid 2). Tumor formed subcutaneously in nude mice after inoculation with HCT-116 cells, observed under oil lens of Leica DM2500 (tumor 1, 2). Green arrows indicate the fiber band.