Figure 7 Cell proliferation analyses are accomplished by green fluorescent protein (GFP)-labeled HCT116 cell imaging. HCT-116 cells transfected with pCDH-CMV-MCS-EF1-copGFP-Puro lentivirus (5×109 transduction units/mL) were cultured in the two-dimensional (2D) plate, silk fibroin (SF)/chitosan (Cs) (1: 1) scaffolds, and SF/Cs/alginate (Alg) (1: 1: 1) scaffolds. The scaffolds and the cells are observed under Nikon ECLIPSE Ts2R microscope and images are taken on day 3 (A–C) and day 7 (D–F). 2D group (A, D), SF/Cs (1: 1) group (B, E), SF/Cs/Alg (1: 1: 1) group (C, F).