10 August 2020>: Lab/In Vitro Research
Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells
Xianhao Su ACF* , Liang Chen AD* , Shanliang Han B , Gengming Niu F , Jun Ren C , Chongwei Ke AG*DOI: 10.12659/MSM.922935
Med Sci Monit 2020; 26:e922935
Figure 8 Fluorescence staining of actin in HCT-116 cells cultured in the extracting liquid of scaffold. F-Actin is marked with fluorescein isothiocyanate (FITC)-DY-554-phalloidin (red). Nuclear counterstain with 4′,6-diamidino-2-phenylindole (DAPI; blue). Two-dimensional (2D) culture system on day 3 (A1), silk fibroin (SF)/chitosan (Cs) (1: 1) scaffold on day 3 (B1), SF/Cs/alginate (Alg) (1: 1: 1) scaffold on day 3 (C1), 2D culture system on day 7 (A2, D1, D2), SF/Cs (1: 1) scaffold on day 7 (B2, E1, E2), SF/Cs/Alg (1: 1: 1) scaffold on day 7 (C2, F1, F2). The green arrow indicates the contractile ring around the nucleus, and the white arrow indicates the contraction ring at the location of the cell mitosis groove.