Figure 8 (A–F) The activity of insulin signaling in Letrozole+HFD, Letrozole, HFD, and control groups of rats. Protein contents in quadriceps femoris muscle (Muscle), omentum majus (Omentum), liver and ovary tissues were examined by Western blot with phospho-specific antibodies for insulin receptor (INSR, Y1361), insulin receptor substrate-1 (IRS-1, Y-612), phosphoinositide 3-kinase (PI3K, Y607), protein kinase B (AKT, Thr308), and extracellular signal-regulated kinases 1 and 2 (ERK1, Y204; ERK2, Y187). Band density was determined using ImageJ software (National Institutes of Health) and expressed as fold changes to each control group after normalization of each protein (band density) with its corresponding loading control (β-actin band density). Data are presented as mean±SEM of 6 rats and were analyzed by one-way ANOVA with Dunnett’s post hoc test. * P 0.05, ** P 0.01, *** P 0.001 compared with control group. NS – not significant.