13 July 2020: Clinical Research
Long-Chain Non-Coding RNA Metastasis-Related Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes the Proliferation and Migration of Human Pulmonary Artery Smooth Muscle Cells (hPASMCs) by Regulating the MicroRNA-503 (miR-503)/Toll-Like Receptor 4 (TLR4) Signal Axis
Meng He 1ABCDEF , Juxin Shen 1ABCDF , Chunyi Zhang 1ABCD , Yefeng Chen 1BCDF , Weisi Wang 2BCD , Kelong Tao 3EG*DOI: 10.12659/MSM.923123
Med Sci Monit 2020; 26:e923123
Figure 2 Silencing of long-chain non-coding RNA metastasis-related lung adenocarcinoma transcript 1 (lncRNA MALAT1) significantly inhibits the proliferation and migration of hPASMCs. (A) Levels of lncRNA MALAT1 expression in hPASMCs with silenced and overexpressed lncRNA MALAT1. (B) Numbers of clones formed by hPASMCs with silenced and overexpressed lncRNA MALAT1. (C) Cell counting kit-8 (CCK-8) assays of the proliferation of hPASMCs with silenced and overexpressed lncRNA MALAT1. (D) Wound healing assay assessing the effect of lncRNA MALAT1 silencing and overexpression on the migration ability of hPASMC cells. (E) Transwell assays assessing the effect of lncRNA MALAT1 silencing and overexpressed on the migration of hPASMC cells. (F) Flow cytometry assays of the effect of lncRNA MALAT1 silencing and overexpression on hPASMC cell apoptosis.* p<0.05, ** p<0.01 compared with the no template control (NC) group.