Figure 4 (A–E) Effects of puerarin on acetylcholine (ACh)-induced release of prostaglandins. High performance liquid chromatography-coupled spectrometry (HPLC-MS) measurement of PGF2α, TXA2, PGE2, and PGD2 in the bath solution of L-NAME (100 μmol/L)-treated mouse carotid arteries with and without exposure to ACh (10 μmol/L, n=5), or to ACh plus puerarin (10 μmol/L, n=3; 50 μmol/L, n=4). The results are means ± standard error of the mean (SEM). * P<0.05 vs. control.