19 February 2021 : Clinical Research
Ultrasound-Targeted Microbubble Destruction Enhances the Inhibitive Efficacy of miR-21 Silencing in HeLa Cells
Shengli Zhao1AE, Jing Xie2AB, Changhua Zhao3C, Wen Cao4D, Yangping Yu5F*DOI: 10.12659/MSM.923660
Med Sci Monit 2021; 27:e923660
Figure 1 UTMD-mediated plasmid transfection of HeLa cells. (A) The influence of ultrasound intensity of 0.5 W/cm2, 1.5 W/cm2, and 2.5 W/cm2 on the transfection rate of the miR-21-EGFP plasmid was determined by flow cytometry. (B) The effect of ultrasound intensity of 0.5 W/cm2, 1.5 W/cm2, and 2.5 W/cm2 on the transfection efficiency of miR-21 inhibitor-EGFP plasmid was determined by flow cytometry. The microbubble concentration was 300 μL/mL, the plasmid concentration was 15 μg/mL, the ultrasound duration was 45 s, the ultrasound frequency was 1 MHz, and the load ratio was 20%. UTMD – ultrasound-targeted microbubble destruction; EGFP – enhanced green fluorescent protein.