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19 February 2021: Lab/In Vitro Research

Ultrasound-Targeted Microbubble Destruction Enhances the Inhibitive Efficacy of miR-21 Silencing in HeLa Cells

Shengli Zhao 1AE* , Jing Xie 2AB* , Changhua Zhao 3C , Wen Cao 4D , Yangping Yu 5F*

DOI: 10.12659/MSM.923660

Med Sci Monit 2021; 27:e923660

Figure 1 UTMD-mediated plasmid transfection of HeLa cells. (A) The influence of ultrasound intensity of 0.5 W/cm2, 1.5 W/cm2, and 2.5 W/cm2 on the transfection rate of the miR-21-EGFP plasmid was determined by flow cytometry. (B) The effect of ultrasound intensity of 0.5 W/cm2, 1.5 W/cm2, and 2.5 W/cm2 on the transfection efficiency of miR-21 inhibitor-EGFP plasmid was determined by flow cytometry. The microbubble concentration was 300 μL/mL, the plasmid concentration was 15 μg/mL, the ultrasound duration was 45 s, the ultrasound frequency was 1 MHz, and the load ratio was 20%. UTMD – ultrasound-targeted microbubble destruction; EGFP – enhanced green fluorescent protein.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750