Figure 4 The expression of cytokines in each group of cultured cells and the NK cell-killing ability. (A) The expression of IL-10 in each culture system is expressed as the mean±SEM, and the experiment was repeated 3 times; *** P<0.001, * P<0.05, ** P<0.01. (B) Expression of IL-12 in each culture system. Data are expressed as the mean±SEM, and the experiment was repeated three times; ** P<0.01. (C) Expression of TNF-α in each culture system. Data are expressed as the mean±SEM, and the experiment was repeated 3 times; ** P<0.01. (D) Expression of IFN-γ in each culture system. Data are expressed as the mean±SEM, and the experiment was repeated 3 times; *** P<0.001, ** P<0.01. (E) YAC-1 cell-killing ability of NK cells isolated from liver tissue from mice in the PBC and CON groups. The experiment was repeated 3 times, and a histogram shows statistically significant differences; *** P<0.001. NK – natural killer; IL – interleukin; SEM – standard error of the mean; TNF – tumor necrosis factor; IFN-γ – interferon-γ.