09 July 2020 : Laboratory Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen1AB, Hongna Guo1AB, Lang Li23A, Dingsu Bao24B, Feng Gao3C, Qiang Li3D, Qi Huang3D, Xin Duan2F, Zhou Xiang2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Figure 6 SNHG15 indirectly regulates KLF4 expression by miR-7. (A) The distribution of SNHG15 in subcellular fractions of chondrocytes was evaluated by qRT-PCR. U6 and GAPDH served as nuclear and cytoplasmic markers, respectively. (B) Correlation analysis results showed that SNHG15 was negatively correlated with miR-7. (C, D) Luciferase reporter assay and RIP confirmed that SNHG15 was a target of miR-7. (E, F) SNHG15 overexpression significantly promoted the expression levels of KLF4 in IL-1β-induced chondrocytes as shown by Western blot. (G, H) MiR-7 significantly reversed the elevated expression of SNHG15 on KLF4 expression in IL-1β-induced chondrocytes as shown by Western blot. **** p<0.0001.