Figure 5 Effects of down-regulating lncRNA SNHG8 on cell damage in hypoxia-induced cardiomyocytes. (A) qRT-PCR assay was performed to measure transfection efficiency. (B–D) Release of CK-MB and cTnI and mitochondrial viability in hypoxia-induced cardiomyocytes were measured by using a K-MB Detection Kit, a cTnI Detection Kit, and a mitochondrial activity assay kit, respectively. Control: cells without any treatment; control-shRNA: H9c2 cells were transfected with control-shRNA for 48 h; SNHG8-shRNA: H9c2 cells were transfected with SNHG8-shRNA for 48 h; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-shRNA: H9c2 cells were transfected with control-shRNA for 48 h under hypoxic conditions; Hypoxia+SNHG8-shRNA: H9c2 cells were transfected with SNHG8-shRNA for 48 h under hypoxic conditions. ** P<0.01 vs. control-shRNA; ## P<0.01 vs. Control group; && P<0.01 vs. Hypoxia+control-shRNA.