09 August 2020>: Clinical Research
Long Non-Coding RNA SNHG8 Plays a Key Role in Myocardial Infarction Through Affecting Hypoxia-Induced Cardiomyocyte Injury
Yue Zhang 1ABCDEFG , Yunfei Bian 2ABCDEG*DOI: 10.12659/MSM.924016
Med Sci Monit 2020; 26:e924016
Figure 6 Effects of downregulating lncRNA SNHG8 on the cell viability and apoptosis in hypoxia-induced cardiomyocytes. (A) Cell viability in hypoxia-induced cardiomyocytes after transfection was determined by MTT assay. (B, C) Flow cytometry analysis was used to detect the apoptotic rate of hypoxia-induced cardiomyocytes. (D, E) Western blotting analysis was used to detect relative expression of cleaved-Caspase3 protein. Control: cells without any treatment; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-shRNA: H9c2 cells were transfected with control-shRNA for 48 h under hypoxic conditions; Hypoxia+SNHG8-shRNA: H9c2 cells were transfected with SNHG8-shRNA for 48 h under hypoxic conditions. ## P<0.01 vs. Control group; && P<0.01 vs. Hypoxia+control-shRNA.