22 July 2020>: Clinical Research
Phospholipase Cɛ Regulates Prostate Cancer Lipid Metabolism and Proliferation by Targeting AMP-Activated Protein Kinase (AMPK)/Sterol Regulatory Element-Binding Protein 1 (SREBP-1) Signaling Pathway
Yongbo Zheng 1ABCDEF* , Jiajia Jin 2ABCDF* , Yingying Gao 3ACF , Chunli Luo 4AG , Xiaohou Wu 1AG* , Jiayu Liu 1ABCDE*DOI: 10.12659/MSM.924328
Med Sci Monit 2020; 26:e924328
Figure 4 Silencing PLCɛ inhibits proliferation and lipid metabolism level of PCa cell lines. (A–D) The lipid droplets content of LNCaP and PC3 cells were analyzed by ORO staining and Nile red staining assays. (E–J) The fluorescence of Ki-67, clone formation, and CCK-8 assays were used to measure the proliferation of PCa cells (400×, 200 μm bar). *** p<0.001, ** p<0.01, ns – no statistical significance; NC – negative control.