22 July 2020 : Laboratory Research
Phospholipase Cε Regulates Prostate Cancer Lipid Metabolism and Proliferation by Targeting AMP-Activated Protein Kinase (AMPK)/Sterol Regulatory Element-Binding Protein 1 (SREBP-1) Signaling Pathway
Yongbo Zheng1ABCDEF, Jiajia Jin2ABCDF, Yingying Gao3ACF, Chunli Luo4AG, Xiaohou Wu1AG, Jiayu Liu1ABCDE*DOI: 10.12659/MSM.924328
Med Sci Monit 2020; 26:e924328
Figure 4 Silencing PLCɛ inhibits proliferation and lipid metabolism level of PCa cell lines. (A–D) The lipid droplets content of LNCaP and PC3 cells were analyzed by ORO staining and Nile red staining assays. (E–J) The fluorescence of Ki-67, clone formation, and CCK-8 assays were used to measure the proliferation of PCa cells (400×, 200 μm bar). *** p<0.001, ** p<0.01, ns – no statistical significance; NC – negative control.