Figure 2 Circ_C16orf62 was expressed at low level in GC cells. (A) Expression of circ_C16orf62 in human normal gastric epithelial cell line (GES-1) and GC cell lines (MKN-74, HGC-27 and AGS) detected by RT-qPCR assay. (B, C) TUBB2A levels in human normal gastric epithelial cell line (GES-1) and GC cell lines (HGC-27 and AGS) were measured by RT-qPCR and western blot assays. (D, E) Relative levels of circ_C16orf62 and C16orf62 mRNA were tested in HGC-27 and AGS cells treated with or without RNase R. (F, G) The cellular localization of circ_C16orf62 in AGS and HGC-27 cells was analyzed by subcellular fractionation assay. * P<0.05.