Figure 4 Interaction between miR-188-3p and FGF1. (A) Binding site between miR-188-3p and the 3′UTR of FGF1 was predicted using TargetScan database. (B) Binding site between miR-188-3p and the 3′UTR of FGF1 was verified using dual-luciferase reporter gene assay. (C) The effect of miR-188-3p on FGF1 expression was examined using qRT-PCR. (D) The effect of miR-188-3p on FGF1 mRNA expression was examined using Western blot analysis. (E) The effect of ox-LDL treatment on FGF1 mRNA expression in VSMCs was examined using qRT-PCR. (F) The effect of ox-LDL treatment on FGF1 expression levels in VSMCs was examined by Western blot analysis. (G) FGF1 mRNA expression in carotid smooth muscle cells from WT and AS mice was measured using qRT-PCR. (H) FGF1 expression in carotid smooth muscle cells from WT and AS mice was measured using qRT-PCR. (I) Expression of FGF1 in ox-LDL-treated VSMCs. * P<0.05, ** P<0.01, and *** P<0.001. In I, ** P<0.01, and *** P<0.001, compared with the 0 mg/L group after 24 h of ox-LDL treatment; &&& P<0.001, compared with the 0 mg/L group after 48 h of ox-LDL treatment.