06 October 2020: Lab/In Vitro Research
miR-188-3p Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Targeting Fibroblast Growth Factor 1 ()
Shaohua Mi 1ACDEG* , Pengfei Wang 2ABCE* , Lejun Lin 3AE*DOI: 10.12659/MSM.924394
Med Sci Monit 2020; 26:e924394
Figure 5 The reversal effect of FGF1 on miR-188-3p function. (A) The effect of co-transfection of miR-188-3p and pcDNA3.1-FGF1 on miR-188-3p expression was examined using qRT-PCR. (B) The effect of co-transfection of miR-188-3p and pcDNA3.1-FGF1 on FGF1 mRNA expression was examined using qRT-PCR. (C) The effect of co-transfection of miR-188-3p and pcDNA3.1-FGF1 on FGF1 expression was examined using Western blot analysis. (D) The proliferation of VSMCs was examined using the MTT assay. (E) The proliferation of VSMCs was examined using the BrdU assay. (F) The migration of VSMCs was examined by the Transwell assay. (G) The apoptosis of VSMCs was examined using flow cytometry. * P<0.05, ** P<0.01, and *** P<0.001.